Anti-RRM1 Monoclonal Antibody

• Catalog Number: orb547853
• Category: Antibodies
• Description: Anti-RRM1 Monoclonal Antibody
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Immunogen: A synthesized peptide derived from human RRM1 Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
• UniProt ID: P23921
• MW: 17 kDa
• Tested applications: FC, ICC, IF, IHC, IP, WB
• Application notes: WB 1:1000-1:5000IHC 1:50-1:200ICC/IF 1:50-1:200IP 1:50FC 1:50. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
• Antibody Type: Primary Antibody
• Clone Number: AFDH-18
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Succinate dehydrogenase [ubiquinone] flavoprotein
• Note: For research use only

Western blot analysis of RRM1 expression in A549 cell lysate using anti-RRM1 antibody (orb547853). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRM1 antigen affinity purified polyclonal antibody (Catalog # orb547853) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90444) with Tanon 5200 system. A specific band was detected for RRM1.