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Anti-RET (Phospho-Y1062) Antibody

Catalog Number: orb304551

DispatchUsually dispatched within 5-10 working days
$ 160.00
Catalog Numberorb304551
CategoryAntibodies
DescriptionRabbit polyclonal antibody to RET (phospho-Y1062)
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Primate, Rat
ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1062 of human RET protein. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetRET
Entrez19713, 5979, 24716
UniProt IDP07949, G3V9H8, P35546
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti CDHF12 antibody, anti CDHR16 antibody, anti P
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-RET (Phospho-Y1062) Antibody

Western blot analysis of RET (Phospho-Y1062) expression in HEK293T (A) whole cell lysates. (Predicted band size: 124 kD; Observed band size: 175 kD)

Anti-RET (Phospho-Y1062) Antibody

Immunohistochemical analysis of RET (Phospho-Y1062) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-RET (Phospho-Y1062) Antibody

Immunofluorescent analysis of RET (Phospho-Y1062) staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Anti-RET (Phospho-Y1062) Antibody

Direct ELISA antibody dose-response curve using Anti-RET (Phospho-Y1062) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.