Anti-RCN3 Antibody

• Catalog Number: orb1879615
• Category: Antibodies
• Description: Rabbit polyclonal antibody to RCN3
• Clonality: Polyclonal
• Tested applications: FC, WB
• Reactivity: Human, Mouse
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human RCN3. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB (1/500 - 1/1000), FC (1/10 - 1/50)
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Entrez: 57333, 52377
• UniProt ID: Q96D15, Q8BH97
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Reticulocalbin-3; EF-hand calcium-binding protein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of RCN3 expression in U251MG (A), U2OS (B), A549 (C), HepG2 (D), mouse lung (E) whole cell lysates. (Predicted band size: 37 kD; Observed band size: 35 kD)

Flow cytometric analysis of CEM cells using Anti-RCN3 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.