RBM25 Antibody

SKU: orb667908

Description

The RBM25 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human RBM25. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: IF, WB
• Dilution range: WB: 1-500-2000
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human RBM25. The exact sequence is proprietary.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

RNPC7; RNA-binding protein 25; Arg/Glu/Asp-rich protein of 120 kDa; RED120; Protein S164; RNA-binding motif protein 25; RNA-binding region-containing protein 7

Western blot analysis of RBM25 expression in HepG2 (A), Jurkat (B), NIH3T3 (C), mouse brain (D) whole cell lysates. (Predicted band size: 17; 32; 33; 100 kD; Observed band size: 120 kD)

Immunofluorescent analysis of RBM25 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

UniProt

P49756, B2RY56

NCBI Gene ID

67039, 58517