Anti-RAGE [2A11]

SKU: orb758837

Description

Mouse monoclonal antibody to RAGE

Research Area

Epigenetics, Immunology

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, FC, IF, IHC, SBR, WB
Reactivity	Human

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	2A11
Immunogen	Human RAGE protein.
Target	RAGE
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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AGER; advanced glycosylation end product-specific receptor; receptor for advanced glycosylation end products

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Flow-cytometry using the Anti-RAGE antibody 2A11. A549 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, shaded line) or the rabbit IgG version of 2A11 (orb758838, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of A549 cells using anti-RAGE antibody 2A11. Immunofluorescence analysis of paraformaldehyde fixed A549 cells stained with the chimeric rabbit IgG version of 2A11 (orb758838) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758838, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (orb256390) followed by Alexa Fluor® 488 secondary antibody.

Western Blot using anti-RAGE antibody 2A11. Human lung lysate (35 µg protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric rabbit IgG version of 2A11 (orb758838) at 0.03 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.