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Catalog Number | orb214491 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to RAD52 |
Target | RAD52 |
Clonality | Polyclonal |
Species/Host | Rabbit |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Porcine, Primate, Rat |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y104 of human RAD52 protein. The exact sequence is proprietary. |
UniProt ID | P43351, P43352 |
Tested applications | IH, WB |
Dilution range | WB: 1-500-1-1000 |
Antibody Type | Primary Antibody |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | anti DNA repair protein RAD52 homolog antibody |
Note | For research use only |
Entrez | 19365, 5893 |
Western blot analysis of RAD52 (Phospho-Y104) expression in HepG2 (A), mouse spleen (B), rat spleen (C) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 46 kD)
Immunohistochemical analysis of RAD52 (Phospho-Y104) staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.