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Anti-Rad21 Rabbit Monoclonal Antibody

Catalog Number: orb547883

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Catalog Numberorb547883
CategoryAntibodies
DescriptionAnti-Rad21 Rabbit Monoclonal Antibody
Species/HostRabbit
ClonalityMonoclonal
Clone NumberCBH-18
Tested applicationsFC, WB
ReactivityHuman, Mouse, Rat
IsotypeRabbit IgG
ImmunogenA synthesized peptide derived from human Rad21
Antibody TypePrimary Antibody
ConcentrationActual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Form/AppearanceLiquid
ConjugationUnconjugated
MW53 kDa
UniProt IDO60216
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesDouble-strand-break repair protein rad21 homolog;
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NoteFor research use only
Application notesWB 1:500-1:1000FC 1:50. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Expiration Date12 months from date of receipt.
Anti-Rad21 Rabbit Monoclonal Antibody

Western blot analysis of Rad21 expression in HeLa cell lysate (orb547883). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD21 monoclonal antibody (Catalog # orb547883) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for RAD21.

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