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Anti-Rad21 Rabbit Monoclonal Antibody

Catalog Number: orb547883

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Catalog Numberorb547883
CategoryAntibodies
DescriptionAnti-Rad21 Rabbit Monoclonal Antibody
ClonalityMonoclonal
Species/HostRabbit
IsotypeRabbit IgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Form/AppearanceLiquid
ConcentrationActual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
ImmunogenA synthesized peptide derived from human Rad21
UniProt IDO60216
MW53 kDa
Tested applicationsFC, WB
Application notesWB 1:500-1:1000FC 1:50. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Antibody TypePrimary Antibody
Clone NumberCBH-18
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesDouble-strand-break repair protein rad21 homolog;
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NoteFor research use only
Anti-Rad21 Rabbit Monoclonal Antibody

Western blot analysis of Rad21 expression in HeLa cell lysate (orb547883). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD21 monoclonal antibody (Catalog # orb547883) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for RAD21.

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