RAB3B Antibody

SKU: orb1473961

Description

The RAB3B Antibody is suitable for FC, IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant fusion protein of human RAB3B. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column.

Images & Validation

• Tested Applications: FC, IHC, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/200), FC (1/10 - 1/50)
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Recombinant fusion protein of human RAB3B. The exact sequence is proprietary.
• Purification: This antibody is purified through a protein G column.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

Ras-related protein Rab-3B

Western blot analysis of RAB3B expression in mouse brain (A), rat brain (B), SW480 (C), human brain (D) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 25 kD)

Immunohistochemical analysis of RAB3B staining in human pancreas formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Flow cytometric analysis of HepG2 cells using Anti-RAB3B Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.

Quick Database Links

UniProt

Q9CZT8, Q63941, P20337

NCBI Gene ID

5865, 81755, 69908