Anti-RAB27 Antibody

• Catalog Number: orb381996
• Category: Antibodies
• Description: Rabbit polyclonal antibody to RAB27A.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse
• Immunogen: Recombinant full length protein of human RAB27
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: RAB27A
• Entrez: 11891, 5873
• UniProt ID: P51159, Q9ERI2
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: RAB27; Ras-related protein Rab-27A; Rab-27; GTP-bi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Simon Heidegger,Florian Stritzke,Sarah Dahl,Juliane Dabler-Plenker,Laura Joachim,Dominik Buschmann,Kaiji Fan,Carolin M. Sauer,Nils Ludwig,Christof Winter,Stefan Enssle,Suqi Li,Markus Perl,Andre Gorgens,Tobias Haas,Erik Thiele Orberg,Sascha Gottert,Catherine Wolfel,Thomas Engleitner,Isidro Cortes-Ciriano,Roland Rad,Wolfgang Herr,Bernd Giebel,Jurgen Ruland,Florian Bassermann,Christoph Coch,Gunther Hartmann,and Hendrik Poeck Targeting nucleic acid sensors in tumor cells to reprogram biogenesis and RNA cargo of extracellular vesicles for T cell-mediated cancer immunotherapy Cell Reports Medicine, 4, 101171 (2023)

PubMed:

Western blot analysis of RAB27 expression in Hela (A), U937 (B), Jurkat (C) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 25 kD)

Immunofluorescent analysis of RAB27 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).