PYK2 Mouse Monoclonal Antibody

SKU: orb1474250

Description

The PYK2 Antibody is suitable for IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against Purified recombinant fragment of PYK2 (aa815-997) expressed in E. Coli. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB (1/500 - 1/1000), IH (1/50 - 1/100)
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of PYK2 (aa815-997) expressed in E. Coli.
• Target: PTK2B
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FAK2; PYK2; RAFTK; Protein-tyrosine kinase 2-beta; Calcium-dependent tyrosine kinase; CADTK; Calcium-regulated non-receptor proline-rich tyrosine kinase; Cell adhesion kinase beta; CAK-beta; CAKB; Focal adhesion kinase 2; FADK 2; Proline-rich tyrosine kinase 2; Related adhesion focal tyrosine kinase; RAFTK

Western blot analysis of PYK2 expression in A549 (A), Ramos (B), Jurkat (C), THP1 (D), Raji (E) whole cell lysates. (Predicted band size: 116 kD; Observed band size: 116 kD)

Immunohistochemical analysis of PYK2 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.139). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

PTK2B

UniProt

Q14289

NCBI Gene ID

2185