PSMD12 Antibody

SKU: orb214470

Description

The PSMD12 Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PSMD12. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics

Images & Validation

• Tested Applications: IHC, WB
• Dilution range: WB: 1-500-1-1000, IHC-P: 1-100-1-200
• Reactivity: Bovine, Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PSMD12. The exact sequence is proprietary.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

26S proteasome non-ATPase regulatory subunit 12; 26S proteasome regulatory subunit RPN5; 26S proteasome regulatory subunit p55

Western blot analysis of PSMD12 expression in HEK293T (A), Hela (B), mouse testis (C), mouse kidney (D), rat testis (E), rat kidney (F), mouse liver (G) whole cell lysates. (Predicted band size: 52 kD; Observed band size: 53 kD)

Immunohistochemical analysis of PSMD12 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

UniProt

Q9D8W5, O00232

NCBI Gene ID

5718, 66997

References

Yvonne Eibach 1 2 3, Silke Kreher 1 2 3, Mareike S Poetsch 1, Ay Lin Kho 4, Ulrich Gaertner 5, Christoph S Clemen 6 7, Rolf Schröder 8, Kai Guo 9 10, Hendrik Milting 11, Benjamin Meder 2 12, Michael Potente 13 14, Manfred Richter 15, Andre Schneider 1 2, Silke Meiners 9 10, Mathias Gaute 4, Thomas Braun The deubiquitinase USP5 prevents accumulation of protein aggregates in cardiomyocytes Sci Adv, (2025)

PubMed: 39841822

Applications

WB

Reactivity

Mouse