Anti-PSMD12 Antibody

• Catalog Number: orb214470
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PSMD12
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PSMD12. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1-500-1-1000, IHC-P: 1-100-1-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PSMD12
• Entrez: 5718, 66997
• UniProt ID: Q9D8W5, O00232
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti 26S proteasome non-ATPase regulatory subunit
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Yvonne Eibach 1 2 3, Silke Kreher 1 2 3, Mareike S Poetsch 1, Ay Lin Kho 4, Ulrich Gaertner 5, Christoph S Clemen 6 7, Rolf Schröder 8, Kai Guo 9 10, Hendrik Milting 11, Benjamin Meder 2 12, Michael Potente 13 14, Manfred Richter 15, Andre Schneider 1 2, Silke Meiners 9 10, Mathias Gaute 4, Thomas Braun The deubiquitinase USP5 prevents accumulation of protein aggregates in cardiomyocytes Sci Adv, (2025)

PubMed: 39841822

Applications

WB

Reactivity

Mouse

Western blot analysis of PSMD12 expression in HEK293T (A), Hela (B), mouse testis (C), mouse kidney (D), rat testis (E), rat kidney (F), mouse liver (G) whole cell lysates. (Predicted band size: 52 kD; Observed band size: 53 kD)

Immunohistochemical analysis of PSMD12 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.