PSMA5 Antibody

SKU: orb1473930

Description

The PSMA5 Antibody is suitable for IF, IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant fusion protein of human PSMA5. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column.

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/10 - 1/50)
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Recombinant fusion protein of human PSMA5. The exact sequence is proprietary.
• Purification: This antibody is purified through a protein G column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

Proteasome subunit alpha type-5; Macropain zeta chain; Multicatalytic endopeptidase complex zeta chain; Proteasome zeta chain

Western blot analysis of PSMA5 expression in MCF7 (A), Neuro2a (B), PC12 (C) whole cell lysates. (Predicted band size: 26 kD; Observed band size: 25 kD)

Immunohistochemical analysis of PSMA5 staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of PSMA5 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Quick Database Links

UniProt

Q9Z2U1, P28066, P34064

NCBI Gene ID

26442, 5686, 29672