PSMA Antibody

SKU: orb43794

Description

Mouse Monoclonal to GCPII.

Images & Validation

• Tested Applications: ICC, IHC-P, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Application Notes:
  Western blotting: Recommended dilution: 1 μg/ml; positive control: LNCaP cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold lysis buffer (1% NP-40). Incubate 30 min on ice. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Both reducing and non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: GCP-04
• Immunogen: Recombinant fragment of human GCPII (amino acids 44-750) produced in S2 cells
• Target: PSMA
• Purification: Purified by protein-A affinity chromatography.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

GCP2, FOLH1, NAALADase I, PGGCP, FGGCP, FGCP,

Immunohistochemistry staining of GCPII in human medulla oblongata by GCP-04 monoclonal antibody. Mag. 40x; positive astrocytes in white matter.

Immunohistochemistry staining of GCPII in human prostate by GCP-04 monoclonal antibody. Mag. 400x; positive epithelium of the prostate glands.

Immunohistochemistry staining of GCPII in porcine kidney by GCP-04 monoclonal antibody. Highly positive proximal glomeruli.

Western blotting analysis of human PSMA using mouse monoclonal antibody GCP-04 on lysates of LNCaP cell line and Jurkat cell line (PSMA non-expressing cell line; negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-PSMA monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody.

Quick Database Links

Gene Symbol

PSMA

UniProt

Q04609

NCBI Gene ID

2346