Anti-PSMA [J591]

• Catalog Number: orb2645800
• Category: Antibodies
• Description: Anti-PSMA [J591]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: J591
• Tested applications: ELISA, FC, IF, IHC, IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: The original antibody was generated by immunizing BALB/c mice with LNCaP cells or a primary culture of PCa epithelial cells.
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: PSMA
• UniProt ID: Q04609
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: PSM; FOLH1; FOLH; FGCP; GCPII; mGCP; NAALAD1; GIG2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using anti-PSMA antibody J591. LNCaP cells were stained with the anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of J591 (orb2645800, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of LNCaP cells with anti-PSMA antibody J591. Immunofluorescence analysis of paraformaldehyde fixed LNCaP cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of J591 (orb2645800) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb2645800, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody