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Catalog Number | orb43636 |
---|---|
Category | Antibodies |
Description | Mouse Monoclonal to Prostate-specific antigen (PSA). |
Clonality | Monoclonal |
Clone Number | A67-B/E3 |
Tested applications | ELISA, IHC-P, WB |
Reactivity | Human |
Isotype | Mouse IgG1 |
Immunogen | 30 kDa protein fraction prepared from human seminal plasma |
Antibody Type | Primary Antibody |
Concentration | 1 mg/ml |
Purity | Purified by protein-A affinity chromatography. |
Conjugation | Unconjugated |
Target | PSA |
Entrez | 354 |
UniProt ID | P07288 |
RRID | AB_10993379 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Prostate-specific antigen (PSA) Read more... |
Note | For research use only |
Application notes | Western blotting: Positive material: seminal plasma, reducing conditions, recommended antibody dilution: 2-5 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemistry staining of prostate (paraffin-embedded sections) with anti-human Prostate-specific antigen (A67-B/E3).
Immunohistochemistry staining of human prostate (paraffin-embedded sections) with anti-PSA (A67-B/E3), 10 µg/ml.
Western blotting analysis of human PSA using mouse monoclonal antibody A67-B/E3 on human seminal plasma under reducing conditions. Nitrocellulose membrane was probed with 5 µg/ml of mouse anti-PSA monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. PSA was detected at approximately 30 kDa.