PSA Antibody

SKU: orb43636

Description

Mouse Monoclonal to Prostate-specific antigen (PSA).

Research Area

Cancer, Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Reactivity: Human
• Application Notes:
  Western blotting: Positive material: seminal plasma, reducing conditions, recommended antibody dilution: 2-5 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: A67-B/E3
• Immunogen: 30 kDa protein fraction prepared from human seminal plasma
• Target: PSA
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Prostate-specific antigen, Kallikrein-3, Semenogelase, Seminin

Immunohistochemistry staining of prostate (paraffin-embedded sections) with anti-human Prostate-specific antigen (A67-B/E3).

Immunohistochemistry staining of human prostate (paraffin-embedded sections) with anti-PSA (A67-B/E3), 10 µg/ml.

Western blotting analysis of human PSA using mouse monoclonal antibody A67-B/E3 on human seminal plasma under reducing conditions. Nitrocellulose membrane was probed with 5 µg/ml of mouse anti-PSA monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. PSA was detected at approximately 30 kDa.

Quick Database Links

Gene Symbol

PSA

UniProt

P07288

NCBI Gene ID

354