Anti-Progesterone Receptor [14B3]

SKU: orb1906345

Description

Anti-Progesterone Receptor [14B3]

Research Area

Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 14B3
• Immunogen: The original antibody was generated by immunizing rabbits with PR protein.
• Target: Progesterone Receptor
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PR; Nuclear receptor subfamily 3 group C member 3

Flow cytometry using anti-Progesterone Receptor antibody 14B3. Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of 14B3 (orb1906344, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of MCF7 cells with anti-Progesterone Receptor antibody 14B3. Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 14B3 (orb1906344) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1906344, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot using anti-Progesterone Receptor antibody 14B3. MCF7 cells lysate (35 µg protein in RIPA buffer) was resolved on an SDS-PAGE gel and blots were probed with the chimeric rabbit version of 14B3 (orb1906344) at 0.01 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and protein was detected by chemiluminescence.

Quick Database Links

Gene Symbol

Progesterone Receptor

UniProt

P06401