Anti-PRMT3 [RAB-C372]

• Catalog Number: orb1089926
• Category: Antibodies
• Description: Human polyclonal antibody to PRMT3
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: RAB-C372
• Tested applications: ChIP, ELISA
• Reactivity: Human
• Isotype: Fab fragment
• Immunogen: This antibody was obtained by recombinant antibody (rAb) phage display recognizing PRMT3 protein under non-denaturing conditions.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: PRMT3
• UniProt ID: O60678
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Heterogeneous nuclear ribonucleoprotein methyltran
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed MCF7 cells with anti-PRMT3 antibody RAB-C372. Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of RAB-C372 (orb1089949) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089949, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-PRMT3 antibody RAB-C372 HEK293(A), HeLa(B), HepG2(C), MCF7(D) and A549(E) cell lysate (35 µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAB-C372 (orb1089949) at 0.001 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.