Anti-PRDX2 [SAIC-34B-124]

• Catalog Number: orb1089982
• Category: Antibodies
• Description: Rabbit monoclonal antibody to PRDX2
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: SAIC-34B-124
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Peptide "LSEDYGVLK", derived from the cellular redox regulator Peroxiredoxin-2, conjugated to KLH.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: PRDX2
• UniProt ID: P32119
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Peroxiredoxin-2;, NKEFB; TDPX1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-PRDX2 antibody SAIC-34B-124. Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of SAIC-34B-124 (orb1089982, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of HeLa cells with anti-PRDX2 SAIC-34B-124. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-34B-124 (orb1089982) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089982, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-PRDX2 antibody SAIC-34B-124. HepG2(A) (0.003 µg/ml), HeLa(B) (0.003 µg/ml), MCF7(C) (0.003 µg/ml), K562(D) (0.003 µg/ml) and LNCaP (E) (0.001 µg/ml) cell lysates (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-34B-124 (orb1089982), before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.