Anti-PRDM9 [RAB-C370]

• Catalog Number: orb1089950
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PRDM9
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: RAB-C370
• Tested applications: ChIP, ELISA, IF
• Reactivity: Human
• Isotype: Human IgG
• Immunogen: This antibody was obtained by recombinant antibody (rAb) phage display recognizing PRDM9 protein under non-denaturing conditions.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: PRDM9
• UniProt ID: Q9NQV7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Histone-lysine N-methyltransferase PRDM9; PR domai
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-PRDM9 antibody RAB-C370. Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of RAB-C370 (orb1089950, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of HEK293 cells with anti-PRDM9 RAB-C370. Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of RAB-C370 (orb1089950) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089950, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Immunofluorescence staining of HeLa cells with anti-PRDM9 RAB-C370. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of RAB-C370 (orb1089950) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089950, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.