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Item 1 of 2
Anti-PRAMEF20/21 Antibody
Catalog Number: orb667785
| Catalog Number | orb667785 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to PRAMEF20/21 |
| Target | PRAMEF20; PRAMEF21 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Immunogen | Recombinant fusion protein of human PRAMEF20/21. The exact sequence is proprietary. |
| UniProt ID | Q5VT98 |
| Tested applications | IH, WB |
| Dilution range | WB: 1-500-2000, IHC-P: 1-50-200 |
| Antibody Type | Primary Antibody |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-PRAME family member 20/21 antibody |
| Note | For research use only |
| Entrez | 627009, 645425, 391001 |
Western blot analysis of PRAMEF20/21 expression in mouse heart (A), rat heart (B) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 55 kD)
Immunohistochemical analysis of PRAMEF20/21 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
