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Anti-PPM1D Purified
Catalog Number: orb1467381
| Catalog Number | orb1467381 |
|---|---|
| Category | Antibodies |
| Description | PPM1D is a Mg2+/Mn2+ dependent protein phosphatase 1D with inducible expression in response to various types of environmental stress. This expression is p53-dependent, and subsequently PPM1D negatively regulates the p53-mediated transcription, thus it suppresses the apoptosis. PPM1D contributes to development of carcinomas, and seems to be a promissing therapeutic target. Amplification of PPM1D is associated with breast cancer. |
| Clonality | Monoclonal |
| Clone Number | 7E11/C5 |
| Tested applications | ELISA, FC, ICC, IHC-P, WB |
| Reactivity | Human, Mouse |
| Isotype | Mouse IgG1 |
| Immunogen | Purified human PPM1D |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Dilution range | Western blotting: Recommended dilution: 1-2 μg/ml. Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining. |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | PPM1D |
| Hazard Information | Non-Toxic |
| Entrez | 8493 |
| UniProt ID | O15297 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | WIP1, Protein phosphatase 1D, JDVS, WIP1, IDDGIP Read more... |
| Note | For research use only |
| Application notes | Western blotting: Recommended dilution: 1-2 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
Western blotting analysis of human PPM1D using mouse monoclonal antibody 7E11/C5 on lysates of U2OS parental cells, expressing both natural (70 kDa, low expression) and C-terminally truncated version (55 kDa, high expression) of PPM1D, and on lysates of MCF7 cells (high PPM1D expression) and RPE cells (low PPM1D expression); reducing conditions.
Separation of MCF-7 cells stained using anti-PPM1D (7E11/C5) purified antibody (concentration in sample 2 µg/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).
