Anti-Podoplanin (MAP tag) [PMab-1]

• Catalog Number: orb2645833
• Category: Antibodies
• Description: Anti-Podoplanin (MAP tag) [PMab-1]
• Target: Podoplanin (MAP tag)
• Clonality: Monoclonal
• Species/Host: Rat
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Immunogen: This antibody was raised by immunising rats with 14-residue synthetic peptide mpp3851, which corresponds to amino acids 38-51 (GDGMVPPGIEDKIT) of the platelet-aggregation-stimulating (PLAG) domain of mouse podoplanin. Spleen cells were then harvested, and fused to P3U1 cells to generate stable hybridomas.
• UniProt ID: Q62011
• Tested applications: Blocking, ELISA, Epitope Tagging, FACS, FC, IHC, WB
• Clone Number: PMab-1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PDPN; Aggrus; Glycoprotein 38; Gp38; OTS-8; Ots8;
• Note: For research use only

ELISA using PMab-1 and Multi Tag protein 2. Binding curves of the mouse IgG1 chimeric version of the anti-MAP antibody PMab-1 (red line) and isotype control (orb256381, anti-fluorescein; orange line) to an ELISA plate coated with Multi Tag protein 2 at a concentration of 5 ug/ml.

Flow-cytometry using the anti-Podoplanin (MAP tag) antibody PMab-1 NIH3T3 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of PMab-1 (orb758777, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of HEK293 cells with Anti-Podoplanin (MAP tag) PMab-1. Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells transfected with Podoplanin (MAP tag) expressing plasmid (A) and non-transfected HEK293 cells (B), permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of PMab-1 (orb758777) (1:200 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue).

Western Blot using anti-Podoplanin (MAP tag) antibody PMab-1 (orb758777) Mouse thymus (A), mouse brain (B), NIH3T3 cells (C) and mouse kidney (D) lysates (35 µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of 1B4 (orb613976) at 0.01 µg/ml, 0.1 µg/ml, 0.001 µg/ml and 0.003 µg/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Western Blot using Anti-Podoplanin (MAP tag) antibody PMab-1. Podoplanin (MAP tag) expressing plasmid transfected (A) and non-transfected (B) HEK293 cells lysate (3 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of PMab-1 (orb758777) at 0.00001 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.