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Anti-PLRP1 Antibody

Catalog Number: orb667737

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb667737
CategoryAntibodies
DescriptionRabbit polyclonal antibody to PLRP1
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenRecombinant fusion protein of human PLRP1. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500-2000
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetPNLIPRP1
Entrez5407, 84028, 18946
UniProt IDQ5BKQ4, P54315, P54316
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesAnti-PLRP1 antibody, anti-Inactive pancreatic lipa
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-PLRP1 Antibody

Western blot analysis of PLRP1 expression in HepG2 (A), mouse liver (B), rat spleen (C) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 52 kD)

Anti-PLRP1 Antibody

Immunohistochemical analysis of PLRP1 staining in rat pancreas formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-PLRP1 Antibody

Immunofluorescent analysis of PLRP1 staining in rat pancreas. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).