PLK1 Mouse Monoclonal Antibody

SKU: orb570316

Description

Anti-PLK1 Antibody (monoclonal, 6D11). Tested in WB applications. This antibody reacts with Human, Monkey.

Research Area

Cancer Biology, Metabolism Research, Neuroscience, Pharmacology & Drug Discovery, Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot, 0.1-0.5μg/ml
• Reactivity: Human, Monkey

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone No.: 6D11
• Immunogen: E.coli-derived human PLK1 recombinant protein (Position: K86-N430). Human PLK1 shares 95.4% and 96.2% amino acid (aa) sequence identity with mouse and rat PLK1, respectively.
• Target: Serine/threonine-protein kinase PLK1
• Molecular Weight: 68 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PLK; PLK 1; PLK1; Polo like kinase 1; STPK13

Western blot analysis of PLK1 using anti-PLK1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates Lane 2: monkey COS-7 whole cell lysates Lane 3: human Raji whole cell lysates Lane 4: human U2OS whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PLK1 antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PLK1 at approximately 68KD. The expected band size for PLK1 is at 68KD.

Quick Database Links

Gene Symbol

Serine/threonine-protein kinase PLK1

UniProt

P53350