PKR Mouse Monoclonal Antibody

SKU: orb1473965

Description

The PKR Antibody [KO/KD Validated] is suitable for IF, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant fusion protein of human PKR. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column.

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB (1/500 - 1/1000), IF/IC (1/10 - 1/50)
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Immunogen: Recombinant fusion protein of human PKR. The exact sequence is proprietary.
• Target: EIF2AK2
• Purification: This antibody is purified through a protein G column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PKR; PRKR; Interferon-induced, double-stranded RNA-activated protein kinase; Eukaryotic translation initiation factor 2-alpha kinase 2; eIF-2A protein kinase 2; Interferon-inducible RNA-dependent protein kinase; P1/eIF-2A protein kinase; Protein kinase RNA-activated; PKR; Tyrosine-protein kinase EIF2AK2; p68 kinase

Western blot analysis of PKR expression in A431 (A), HepG2 (B), SHSY5Y (C) whole cell lysates. (Predicted band size: 62 kD; Observed band size: 68 kD)

Immunofluorescent analysis of PKR staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

EIF2AK2

UniProt

P19525

NCBI Gene ID

5610