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Anti-PKN1/2 (Phospho-T774/816) Antibody

Catalog Number: orb515530

DispatchUsually dispatched within 5-10 working days
$ 160.00
Catalog Numberorb515530
CategoryAntibodies
DescriptionRabbit polyclonal antibody to PKN1 (phospho-T774/816)
TargetPKN1; PKN2
ClonalityPolyclonal
Species/HostRabbit
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
PurificationThe antibody was purified by immunogen affinity chromatography.
ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T774/816 of human PKN1/2 protein. The exact sequence is proprietary.
UniProt IDQ16513, Q16512, O08874, Q8BWW9, P70268, Q63433
Tested applicationsWB
Dilution rangeWB: 1:500-1000
Antibody TypePrimary Antibody
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesPKN1; PAK1; PKN; PRK1; PRKCL1; Serine/threonine-pr
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NoteFor research use only
Entrez5585, 5586, 109333, 320795, 29355
Anti-PKN1/2 (Phospho-T774/816) Antibody

Western blot analysis of PKN1/2 (Phospho-T774/816) expression in HCT116 (A) whole cell lysates. (Predicted band size: 103; 112 kD; Observed band size: 130 kD)

Anti-PKN1/2 (Phospho-T774/816) Antibody

Direct ELISA antibody dose-response curve using Anti-PKN1/2 (Phospho-T774/816) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.