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Anti-PKC theta (Phospho-S695) Antibody

Catalog Number: orb338830

DispatchUsually dispatched within 5-10 working days
$ 190.00
Catalog Numberorb338830
CategoryAntibodies
DescriptionRabbit polyclonal antibody to PRKCQ
TargetPRKCQ
ClonalityPolyclonal
Species/HostRabbit
ConjugationUnconjugated
ReactivityHuman
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S695 of human PKC theta protein. The exact sequence is proprietary.
UniProt IDQ04759
Tested applicationsIH, WB
Dilution rangeWB: 1:500-1000, IHC-P: 1:100-200
Antibody TypePrimary Antibody
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesanti PRKCT antibody, anti Protein kinase C theta t
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NoteFor research use only
Entrez5588
Anti-PKC theta (Phospho-S695) Antibody

Western blot analysis of PKC theta (Phospho-S695) expression in Jurkat PMA-treated (A), HEK293T PMA-treated (B) whole cell lysates. (Predicted band size: 81 kD; Observed band size: 80 kD)

Anti-PKC theta (Phospho-S695) Antibody

Immunohistochemical analysis of PKC theta (Phospho-S695) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.