Anti-PKC theta Antibody

• Catalog Number: orb315665
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PKC theta
• Target: PRKCQ
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PKC theta. The exact sequence is proprietary.
• UniProt ID: Q04759
• Tested applications: IH, WB
• Dilution range: WB: 1-500:1000, IHC-P: 1-100:200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti PRKCT antibody, anti Protein kinase C theta t
• Note: For research use only
• Entrez: 5588

Western blot analysis of PKC theta expression in MCF7 (A), Jurkat (B) whole cell lysates. (Predicted band size: 81 kD; Observed band size: 78 kD)

Immunohistochemical analysis of PKC theta staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.