PKC epsilon (Phospho-S729) Rabbit Polyclonal Antibody

SKU: orb315664

Description

The PKC epsilon (Phospho-S729) Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S729 of human PKC epsilon protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics, Immunology

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1-500:1000, IF/ICC: 1-100:500
• Reactivity: Human, Mouse, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S729 of human PKC epsilon protein. The exact sequence is proprietary.
• Target: PRKCE
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PKCE; Protein kinase C epsilon type; nPKC-epsilon

Western blot analysis of PKC epsilon (Phospho-S729) expression in HEK293T (A), H1792 (B) whole cell lysates. (Predicted band size: 83 kD; Observed band size: 82 kD)

Immunofluorescent analysis of PKC epsilon (Phospho-S729) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

PRKCE

UniProt

Q02156, P16054, P09216

NCBI Gene ID

5581, 29340, 18754

Di Pietro, Paola et al. Targeting the ASMase/S1P pathway protects from sortilin-evoked vascular damage in hypertension J Clin Invest, 132, e146343 (2022)

PubMed: 35104805