Anti-PI3K p85 alpha Antibody

• Catalog Number: orb1474611
• Category: Antibodies
• Description: Rabbit monoclonal antibody to PI3K p85 alpha
• Target: PIK3R1
• Clonality: Monoclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Hamster, Human, Rat
• Form/Appearance: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Buffer/Preservatives: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: A synthetic peptide of human PI 3 Kinase p85 alpha
• UniProt ID: P27986, Q63787
• Tested applications: IF, IP, WB
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50)
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: GRB1; Phosphatidylinositol 3-kinase regulatory sub
• Note: For research use only
• Entrez: 25513, 5295

Western blot analysis of PI3K p85 alpha expression in Jurkat (A), C6 (B), CHOK1 (C) whole cell lysates. (Predicted band size: 84 kD; Observed band size: 84 kD)

Immunofluorescent analysis of PI3K p85 alpha staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.