Anti-PHOX2B Monoclonal Antibody

• Catalog Number: orb547978
• Category: Antibodies
• Description: Anti-PHOX2B Monoclonal Antibody. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse.
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Purification: Affinity-chromatography
• Immunogen: A synthesized peptide derived from human PHOX2B Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum-response element.
• UniProt ID: Q99453
• MW: 90 kDa
• Tested applications: ICC, IF, IHC, WB
• Application notes: WB 1:500-1:2000IHC 1:100-1:500ICC/IF 1:50-1:200. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
• Antibody Type: Primary Antibody
• Clone Number: AEFG-16
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD276 antigen; 4Ig-B7-H3; B7 homolog 3; B7-H3; Cos
• Note: For research use only

Western blot analysis of PHOX2B expression in Neuro-2a cell lysate using anti-PHOX2B antibody (orb547978). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHOX2B antigen affinity purified polyclonal antibody (Catalog # orb547978) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90444) with Tanon 5200 system. A specific band was detected for PHOX2B.