Phospho-POLR2A (S2) Rabbit Monoclonal Antibody

SKU: orb548620

Description

Anti-Phospho-POLR2A (S2) Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Epigenetics & Chromatin, Molecular Biology

Images & Validation

• Tested Applications: FC, ICC, IF, IHC, IP, WB
• Dilution range: WB 1:500-2000 IHC 1:50-200 ICC/IF 1:50-200 IP 1:20 FC 1:20
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: AH-16
• Immunogen: A synthesized peptide derived from human POLR2A
• Target: DNA-directed RNA polymerase II subunit RPB1
• Molecular Weight: 80 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml rAlbumin.
• Concentration: 0.5mg/ml
• Disclaimer: For research use only

Alternative Names

DNA-ed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; 2.7.7.6; DNA-ed RNA polymerase II subunit A; DNA-ed RNA polymerase III largest subunit; RNA-ed RNA polymerase II subunit RPB1; 2.7.7.48; POLR2A; POLR2

Western blot analysis of Phospho-POLR2A (S2) expression in MCF7 cell lysate treated with Lambda Phosphatase (orb548620) (1) ; MCF7 cell lysate (2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR2A monoclonal antibody (Catalog # orb548620) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for POLR2A.

Quick Database Links

Gene Symbol

DNA-directed RNA polymerase II subunit RPB1

UniProt

P24928