Phospho-PDGF Receptor beta (Y740) PDGFRB Rabbit Monoclonal Antibody

SKU: orb548580

Description

Anti-Phospho-PDGF Receptor beta (Y740) PDGFRB Rabbit Monoclonal Antibody. Tested in WB, ICC/IF applications. This antibody reacts with Mouse, Rat.

Images & Validation

• Tested Applications: ICC, IF, WB
• Reactivity: Mouse, Rat
• Application Notes:
  WB 1:500-2000ICC/IF 1:50-200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: FDF-16
• Immunogen: A synthesized peptide derived from human Phospho-PDGF Receptor beta (Y740)
• Molecular Weight: 100 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Concentration: 0.5mg/ml
• Disclaimer: For research use only

Alternative Names

Platelet-derived growth factor receptor beta; PDGF-R-beta; PDGFR-beta; 2.7.10.1; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; Platelet-derived growth factor receptor 1; PDGFR-1; CD140b; PDGFRB; PDGFR, PDGFR1

Western blot analysis of Phospho-PDGF Receptor beta (Y740) expression in NIH 3T3 treated with PDGF (orb548580). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDGFRB monoclonal antibody (Catalog # orb548580) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for PDGFRB.

Quick Database Links

UniProt

P09619