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Phospho-Erk1 + Erk2 MAPK3 Rabbit Monoclonal Antibody
SKU: orb548583
Description
Research Area
Cell Biology, Neuroscience, Protein Biochemistry, Signal Transduction, Stem Cell & Developmental Biology
Images & Validation
−Item 1 of 1
| Tested Applications | IP, WB |
|---|---|
| Dilution Range | WB 1:500-2000 IP 1:20 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Clone No. | BIH-13 |
| Immunogen | A synthesized peptide derived from human Phospho-ERK1 (T202/Y204) + ERK2 (T185/Y187) |
| Molecular Weight | 50-70 kDa |
| Purification | Affinity-chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required. |
| Concentration | 0.5mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; 2.7.11.24; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p44-ERK1; MAPK3; ERK1, PRKM3
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−Phospho-Erk1 + Erk2 MAPK3 Rabbit Monoclonal Antibody [orb548584]
FC, ICC, IF, IHC, WB
Human
Rabbit
Monoclonal
Unconjugated
100 μl

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Western blot analysis of Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) expression in A431 cell lysate treated with EGF.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IP
Immunoprecipitation
Phospho-Erk1 + Erk2 MAPK3 Rabbit Monoclonal Antibody (orb548583)
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