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Anti-Phospho-Cyclin E1 (T77) CCNE1 Rabbit Monoclonal Antibody
Catalog Number: orb548608
| Catalog Number | orb548608 |
|---|---|
| Category | Antibodies |
| Description | Anti-Phospho-Cyclin E1 (T77) CCNE1 Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human. |
| Clonality | Monoclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Liquid |
| Concentration | Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
| Purification | Affinity-chromatography |
| Immunogen | A synthesized peptide derived from human Cyclin E1 |
| UniProt ID | P24864 |
| MW | 185 kDa |
| Tested applications | ICC, IF, IHC, WB |
| Application notes | WB 1:1000-1:2000IHC 1:50-1:200ICC/IF 1:50-1:200 |
| Antibody Type | Primary Antibody |
| Clone Number | GHC-3 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | G1/S-specific cyclin-E1; CCNE1; CCNE |
| Note | For research use only |
Western blot analysis of Phospho-Cyclin E1 (T77) expression in (1) JAR cell treated with Lambda Phosphatase lysate; (2) JAR cell lysate (orb548608). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCNE1 monoclonal antibody (Catalog # orb548608) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for CCNE1.
