Phospho-c-Jun (S63) Rabbit Monoclonal Antibody

SKU: orb548642

Description

Anti-Phospho-c-Jun (S63) Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB 1:500-2000IHC 1:50-200ICC/IF 1:50-200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: IGA-10
• Immunogen: A synthesized peptide derived from human Phospho-c-Jun (S63)
• Molecular Weight: 43 kDa
• Purification: Affinity-chromatography

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Concentration: 0.5mg/ml
• Disclaimer: For research use only

Alternative Names

Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39; JUN

Western blot analysis of Phospho-c-Jun (S63) expression in NIH/3T3 cell lysate treated with Anisomycin (orb548642). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Phospho-c-Jun (S63) monoclonal antibody (Catalog # orb548642) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system.

IHC analysis of Phospho-c-Jun (S63) using anti-Phospho-c-Jun (S63) antibody (orb548642) on human breast carcinoma. Phospho-c-Jun (S63) was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Phospho-c-Jun (S63) Antibody (orb548642) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.

IF analysis of immunocytochemical section of HeLa cells treated with anisomycin using anti-Phospho-c-Jun (S63) antibody (orb548642) Phospho-c-Jun (S63) was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (orb90553) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-Phospho-c-Jun (S63) Antibody (orb548642) overnight at 4 °C. DyLight®488 Conjugated Goat AntiRabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 °C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Quick Database Links

UniProt

P05412