Phospho-ATF2/ATF2 Rabbit Monoclonal Antibody

SKU: orb548618

Description

Anti-Phospho-ATF2 (T71) Rabbit Monoclonal Antibody. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human.

Research Area

Epigenetics & Chromatin, Immunology & Inflammation

Images & Validation

• Tested Applications: ICC, IF, IP, WB
• Dilution Range: WB 1:500-2000 ICC/IF 1:50-200 IP 1:20
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: FOO-1
• Immunogen: A synthesized peptide derived from human Phospho-ATF2 (T71)
• Target: Cyclic AMP-dependent transcription factor ATF-2
• Molecular Weight: 80 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: 0.5mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cyclic AMP-dependent transcription factor ATF-2; cAMP-dependent transcription factor ATF-2; 2.3.1.48; Activating transcription factor 2; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding protein 2; HB16; Histone acetyltransferase ATF2; cAMP response element-binding protein CRE-BP1; ATF2; CREB2, CREBP1

Western blot analysis of Phospho-ATF2 (T71) expression in (1) HeLa cell lysate; (2) HeLa cell lysate treated with Anisomycin.

ICC staining of Phospho-ATF2 (T71) in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS.

Quick Database Links

Gene Symbol

Cyclic AMP-dependent transcription factor ATF-2

UniProt

P15336