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Anti-PHKG1 Antibody

Catalog Number: orb214391

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb214391
CategoryAntibodies
DescriptionRabbit polyclonal antibody to PHKG1
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, WB
ReactivityHuman, Mouse, Rat
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the center region of human PHKG1. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500-1-1000
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetPHKG1
Entrez5260, 29353, 18682
UniProt IDP07934, Q16816, P13286
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti PHKG antibody, anti Phosphorylase b kinase ga
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-PHKG1 Antibody

Western blot analysis of PHKG1 expression in SHSY5Y (A), EC9706 (B), HCT116 (C), H9C2 (D), BV2 (E), rat brain (F) whole cell lysates. (Predicted band size: 45 kD; Observed band size: 55 kD)

Anti-PHKG1 Antibody

Immunofluorescent analysis of PHKG1 staining in PANC1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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