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Description
Research Area
Cancer Biology, Cardiovascular Research, Cell Biology, Epigenetics & Chromatin, Metabolism Research
Images & Validation
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Item 1 of 1
| Tested Applications | IP, WB |
|---|---|
| Dilution Range | WB 1:500-2000 IP 1:20 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Clone No. | BCEGHX7 |
| Immunogen | A synthesized peptide derived from human PHD2 / prolyl hydroxylase Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. |
| Target | Egl nine homolog 1 |
| Molecular Weight | 52 kDa |
| Purification | Affinity-chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required. |
| Concentration | 0.5mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Protein S100-A8; Calgranulin-A; Calprotectin L1L subunit; Cystic fibrosis antigen; CFAG; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8; MRP-8; p8; S100 calcium-binding protein A8; Urinary stone protein band A; Protein S100-A8, N-terminally processed; S100A8; CAGA, CFAG, MRP8
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PHD2 / prolyl hydroxylase Rabbit monoclonal antibody [orb2297800]
IP, WB
Human
Rabbit
Monoclonal
Unconjugated
25 μl, 50 μl, 100 μl
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Quick Database Links
Gene Symbol
Egl nine homolog 1
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IP
Immunoprecipitation
Available Sizes
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Free Secondary Antibody (20 ul)0/0
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Usually dispatched within 2-4 weeks