PGK1 Antibody [KO/KD Validated]

• Catalog Number: orb1474128
• Category: Antibodies
• Description: The PGK1 Antibody [KO/KD Validated] is suitable for FC, IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary.
• UniProt ID: P00558, P09411
• Tested applications: FC, IHC, WB
• Dilution range: WB (1/500 - 1/2000), IH (1/50 - 1/100), FC (1/50 - 1/100)
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PGKA; Phosphoglycerate kinase 1; Cell migration-in
• Note: For research use only
• Entrez: 5230, 18655

Western blot analysis of PGK1 expression in HEK293T (A), A431 (B), mouse brain (C) whole cell lysates. (Predicted band size: 44 kD; Observed band size: 44 kD)

Immunohistochemical analysis of PGK1 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Overlay histogram showing Jurkat cells stained with Anti-PGK1 Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by Anti-PGK1 Antibody for 60 min at 37 °C. The secondary antibody used was Goat Anti-Mouse IgG (H&L) - AF488 at 1/200 dilution for 40 min at 37 °C. Isotype control antibody (blue line) was mouse IgG2a (1μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.