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Anti-PGHS-2 Antibody

Catalog Number: orb214476

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb214476
CategoryAntibodies
DescriptionRabbit polyclonal antibody to PTGS2
TargetPTGS2
ClonalityPolyclonal
Species/HostRabbit
ConjugationUnconjugated
ReactivityHuman, Porcine, Sheep
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PGHS-2. The exact sequence is proprietary.
UniProt IDP35354
Tested applicationsIF, IH, WB
Dilution rangeWB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500
Antibody TypePrimary Antibody
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesanti COX2 antibody, anti Prostaglandin G/H synthas
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NoteFor research use only
Entrez5743
Anti-PGHS-2 Antibody

Western blot analysis of PGHS-2 expression in A549 (A) whole cell lysates. (Predicted band size: 68 kD; Observed band size: 70 kD)

Anti-PGHS-2 Antibody

Immunohistochemical analysis of PGHS-2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-PGHS-2 Antibody

Immunofluorescent analysis of PGHS-2 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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