Anti-PGAP1 Antibody

• Catalog Number: orb1879144
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PGAP1
• Clonality: Polyclonal
• Tested applications: FC, IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human PGAP1. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/10 - 1/50), FC (1/10 - 1/30)
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Entrez: 241062, 80055, 316400
• UniProt ID: Q765A7, Q3UUQ7, Q75T13
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Alternative names: GPI inositol-deacylase; Post-GPI attachment to pro
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PGAP1 expression in SHSY5Y (A), Hela (B), 293 (C), mouse kidney (D), mouse testis (E), rat kidney (F) whole cell lysates. (Predicted band size: 105 kD; Observed band size: 105 kD)

Immunofluorescent analysis of Anti-PGAP1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Flow cytometric analysis of Hela cells using Anti-PGAP1 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.