PERP Monoclonal Antibody

SKU: orb548263

Description

PERP Monoclonal Antibody

Research Area

Cancer Biology, Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution range: WB 1:500-2000 ICC/IF 1:50-200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: AFDD-16
• Immunogen: A synthesized peptide derived from human PERP
• Target: p53 apoptosis effector related to PMP-22
• Molecular Weight: 46 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml rAlbumin.
• Concentration: 0.5mg/ml
• Disclaimer: For research use only

Alternative Names

Perilipin-1; Lipid droplet-associated protein; PLIN1; PERI, PLIN

Western blot analysis of PERP expression in A431 cell lysate using anti-PERP antibody (orb548263). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PERP antigen affinity purified polyclonal antibody (Catalog # orb548263) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system.

Quick Database Links

Gene Symbol

p53 apoptosis effector related to PMP-22

UniProt

Q96FX8