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Anti-Perforin APC
Catalog Number: orb1742786
| Catalog Number | orb1742786 |
|---|---|
| Category | Antibodies |
| Description | Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders. |
| Target | Perforin |
| Clonality | Monoclonal |
| Isotype | Mouse IgG2b kappa |
| Conjugation | APC |
| Reactivity | Bovine, Human |
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Purification | Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Immunogen | purified granules from human YT lymphoma cell line |
| UniProt ID | P14222 |
| Tested applications | FC |
| Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining. |
| Clone Number | dG9 |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Alternative names | PRF1, P1, PFP, HPLH2 |
| Note | For research use only |
| Entrez | 5551 |
Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD56 (LT56) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and intracellular staining using anti-Perforin (dG9) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
Separation of human Perforin positive CD56 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-Perforin (dG9) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-Perforin (dG9) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
