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Anti-PDE8A Purified
Catalog Number: orb154411
| Catalog Number | orb154411 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody to PDE8a which is a member of the cyclic nucleotide phosphodiesterase family and PDE8 subfamily. PDE8B is expressed specifically and abundantly in the thyroid gland and shares 65% sequence identity with PDE8A. It highly expressed in testis, ovary, small intestine and colon. It may be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development. |
| Clonality | Monoclonal |
| Clone Number | EM-52 |
| Tested applications | WB |
| Reactivity | Human |
| Isotype | Mouse IgG1 kappa |
| Immunogen | Human PDE8a |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | PDE8A |
| Entrez | 5151 |
| UniProt ID | O60658 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | Anti-HsT19550 antibody Read more... |
| Note | For research use only |
| Application notes | Western blotting: Recommended dilution: 1-2 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
Western bloting analysis of human PDE8A using mouse monoclonal antibody EM-52 on lysates of HeLa cell line and TF-1 cell line (negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody anti-PDE8A followed by Alexa Fluor® 790-conjugated goat-anti-mouse secondary antibody. A specific band was detected for PDE8A at approximately 95 kDa.
