PDE6D Rabbit Polyclonal Antibody

SKU: orb667642

Description

The PDE6D Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human PDE6D. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB: 1-500-2000, IF: 1-50-200
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human PDE6D. The exact sequence is proprietary.
• Target: PDE6D
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PDED; Retinal rod rhodopsin-sensitive cGMP 3' 5'-cyclic phosphodiesterase subunit delta; GMP-PDE delta; Protein p17

Western blot analysis of PDE6D expression in HT29 (A), mouse brain (B), mouse kidney (C) whole cell lysates. (Predicted band size: 17 kD; Observed band size: 14-15 kD)

Immunofluorescent analysis of PDE6D staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

PDE6D

UniProt

O43924, O55057

NCBI Gene ID

5147, 18582