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PDCD4 (Phospho-S457) Antibody
SKU: orb256744
Description
Images & Validation
−Item 1 of 2
| Tested Applications | IHC, WB |
|---|---|
| Dilution range | WB: 1:500-1000, IHC-P: 1:100-200 |
| Reactivity | Bovine, Gallus, Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S457 of human PDCD4 protein. The exact sequence is proprietary. |
| Purification | The antibody was purified by immunogen affinity chromatography. |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Disclaimer | For research use only |
Alternative Names
−H731; Programmed cell death protein 4; Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a
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Western blot analysis of PDCD4 (Phospho-S457) expression in HeLa (A), Jurkat (B) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 60 kD)
Immunohistochemical analysis of PDCD4 (Phospho-S457) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
PDCD4 (Phospho-S457) Antibody (orb256744)
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