PDCD4 Rabbit Polyclonal Antibody

SKU: orb1880429

Description

The PDCD4 Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human PDCD4. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB (1/500 - 1/1000), IH (1/50 - 1/100)
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human PDCD4. The exact sequence is proprietary.
• Target: PDCD4
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

H731; Programmed cell death protein 4; Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a

Western blot analysis of PDCD4 expression in Hela (A), Jurkat (B), HepG2 (C), 293 (D), HL60 (E), mouse thymus (F) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 55 kD)

Immunohistochemical analysis of PDCD4 staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

PDCD4

UniProt

Q53EL6, Q9JID1, Q61823

NCBI Gene ID

18569, 27250, 64031