Anti-PD-L1 [332M7]

• Catalog Number: orb1152460
• Category: Antibodies
• Description: Rabbit monoclonal antibody to PD-L1
• Target: PD-L1
• Clonality: Monoclonal
• Species/Host: Human
• Isotype: Human IgG
• Conjugation: Unconjugated
• Reactivity: Human, Monkey, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: The original parental antibody 332M1 was generated by immunizing BALB/c mice with recombinant human PD-L1. Later on the humanized version of 332M1 was generated (332M7) by grafting the CDRs of the original mouse antibody onto human framework regions.
• UniProt ID: Q9NZQ7
• Tested applications: Blocking, FC
• Clone Number: 332M7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD274; PDL1; B7-H1; B7H1; B7 homolog 1; hPD-L1; PD
• Note: For research use only

Flow cytometry using the Anti-PD-L1 antibody 332M7. Paraformaldehyde fixed MDA-MB-231 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of 332M7 (orb1152460, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of MDA-MB-231 cells with anti-PD-L1 332M7. Immunofluorescence analysis of paraformaldehyde fixed MDA-MB-231 cells stained with the chimeric rabbit IgG version of 332M7 (orb1152460) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1152460, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.