Anti-PD-1 [5C4.B8 (Nivolumab)]

• Catalog Number: orb1821656
• Category: Antibodies
• Description: Anti-PD-1 [5C4.B8 (Nivolumab)]
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: 5C4.B8 (Nivolumab)
• Tested applications: Blocking, FC, IHC, SBR
• Reactivity: Human, Monkey
• Isotype: Human IgG1
• Immunogen: mAb PD1.5 was prepared by immunizing IgH and IgK knock-out transgenic mice possessing a human immunoglobulin (heavy chain) minilocus with recombinant human PD-1-Fc protein consisting of the extracellular domain of PD-1 (amino acids 1–167) and the Fc portion of human IgG1, and Chinese hamster ovary (CHO) cells expressing human PD-1. Nivolumab was generated by grafting the variable regions of PD1.5 onto human kappa and IgG4 constant regions containing an S228P mutation (prevents Fab arm exchange with endogenous IgG4 antibodies).
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: PD-1
• UniProt ID: Q15116
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD279; programmed death-1; programmed death 1 ; PD
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using anti-PD-1 antibody Nivolumab Cynomolgus monkey lymphocytes were stained with an isotype control (orb256390, red) or the rabbit-chimeric version of Nivolumab (orb1821655, blue) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FlowJo single-cell flow cytometer.

Flow-cytometry using anti-PD-1 antibody Nivolumab Rhesus monkey lymphocytes were stained with an isotype control (orb256390, red) or the rabbit-chimeric version of Nivolumab (orb1821655, blue) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FlowJo single-cell flow cytometer.